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KMID : 0848120080330040131
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International Journal of Oral Biology
2008 Volume.33 No. 4 p.131 ~ p.135
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Effects of substance P on mineralization markers and heme oxygenase-1 Expression in human immortalized periodontal ligament cells
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Cho You-Min
Chun Sang-Woo
Kim Eun-Cheol
Kang Kyung-Hwa
Suh Chung-Hwan
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Abstract
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Substance P (SP) is known to be expressed in the nerve fibers of dental pulp and periodontal tissues. It was recently
reported that SP expression increased in response to orthodontic force. In the present study, we investigated the effect of SP on expression of mineralization markers and heme oxygenase-1 (HO-1) in human immortalized periodontal ligament (IPDL) cells. Cell viability was measured using a 3,4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) assay. The expression of mineralization markers, including alkaline phosphatase (ALP), osteonectin (ON) and bone sialoprotein (BSP), and heme oxygenase-1 (HO-1) was assessed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. SP did not significantly change human IPDL cell viability, with the exception of the 24 hour treatment group. Treatment of human IPDL cells with 10 -10 to 10 -4 M SP upregulated mineralization marker and HO-1 expression in a time- and concentration-dependent manner. Our results suggest that SP may modulate osteoblastic cell differentiation of human IPDL cells through a mechanism involving HO-1 expression.
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KEYWORD
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Periodontal ligament cell, Substance P, Mineralization marker, Heme oxygenase-1
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